Research Paper Volume 11, Issue 20 pp 8860—8878

Long non-coding SBF2-AS1 acting as a competing endogenous RNA to sponge microRNA-142-3p to participate in gemcitabine resistance in pancreatic cancer via upregulating TWF1

Figure 5. Chemosensitivity of parental cells and corresponding drug-resistant cells as well as expression of SBF2-AS1 in parental cells. (A) Detection of gemcitabine sensitivity in AsPC-1/GEM and PAN-1/GEM cells by MTT assay. (B) Detection of transfection efficiency of SBF2-AS1 overexpression plasmid by RT-qPCR. (C) Detection of gemcitabine sensitivity in AsPC-1 and PANC-1 cells by MTT assay. Repetitions = 3; Data was analyzed using the t test or one-way ANOVA. * P < 0.05 vs the same concentration of the blank group or the control group.