Research Paper Volume 11, Issue 18 pp 7996—8014

Differential effects of extracellular vesicles from aging and young mesenchymal stem cells in acute lung injury

Figure 1. Aging MSCs showed no beneficial effects in LPS-induced lung injury compared with young MSCs. Mice were divided into 4 group: control, LPS, LPS + young MSCs (1 × 106 cells), LPS + aging MSCs (1 × 106 cells). MSCs were administered intravenously 30 min after intratracheal LPS treatment. (A) Lung tissue was harvested at 48 h after LPS treatment and stained with H&E and visualized at x200 magnification (scale bar: 50 μm). (B and C) Protein level, total cell count, and neutrophil count in the BAL were examined at 48 h after LPS treatment to evaluate inflammatory response. Data are presented as mean ± SEM, n = 7–10. *p < 0.05, **p < 0.01, *** p < 0.001. (D) Cytokine levels (IL-1β and IL-10) in the BAL at 48 h after LPS treatment were assayed via ELISA. Data are presented as mean ± SEM, n = 4–7. *p < 0.05, **p < 0.01, *** p < 0.001.