Figure 2. EVs derived from young and aging human adipose-derived MSCs showed similar characteristics. (A) Morphology of the isolated young and aging MSC-EVs as imaged by transmission electron microscopy (scale bar: 0.5 μm). (B–D) Size and particle concentrations of isolated young and aging MSC-EVs were examined via nanoparticle tracking analysis. Data are presented as mean ± SEM, n = 4–7. (E) Markers for MSC-EVs were assayed by Western blot analysis, n = 3.