Figure 7. Aging MSC-EVs were less readily internalized by macrophages compared with young MSC-EVs. (A–B) BMDMs (8 × 105 cells) were incubated with CD63-labelled aging or young MSC-EVs (10 μg) in a 12-well plate for 6 h in the presence or absence of LPS and stained with PE-Cy7-conjugated anti-mouse F4/80 antibody. Data were analyzed via flow cytometry. Data are presented as mean ± SEM, n = 6. **p < 0.01, *** p < 0.001. MΦ = Macrophages.