Research Paper Volume 11, Issue 20 pp 8892—8910

TGF-β receptor inhibitor LY2109761 enhances the radiosensitivity of gastric cancer by inactivating the TGF-β/SMAD4 signaling pathway

Figure 2. Different biological characteristics in radioresistant RR and parental GC cells. (A) Clonogenic survival assay: the ability of colony formation between RR and parental GC cells after a range of radiation doses. (B) Flow cytometry analysis: ROS levels between RR and parental GC cells. (C) Western blot analysis: representative results of the TGF-β1, TGF-β2, SMAD4, E-CA, N-CA, IL-1β, IL-6, PD-L1 and γ-H2AX expressions in RR and parental GC cells. (D) Sphere formation assays: captured images of sphere formation assays in RR and parental GC cells. (E) Flow cytometry analysis: the expressions of the CSC markers CD24 and CD133 between SGC-7901 and SGC-7901-R cells. (F) Flow cytometry analysis: cell apoptosis between SGC-7901 and SGC-7901-R cells after irradiation treatment. (G) Cell migration assay: captured images of wound healing assay of SGC-7901 and SGC-7901-R cells, columns indicated the percentage of wound healing width of SGC-7901 and SGC-7901-R cells. (H) Cell invasion assay: captured images of transwell assay of SGC-7901 and SGC-7901-R cells, columns indicated the invaded cell percentage of SGC-7901 and SGC-7901-R cells. All data represent three independent experiments, mean ± SEM, **P<0.01, ***P<0.001, ****P<0.0001.