Research Paper Volume 11, Issue 21 pp 9264—9279

Dopaminergic neuron injury in Parkinson’s disease is mitigated by interfering lncRNA SNHG14 expression to regulate the miR-133b/ α-synuclein pathway

Figure 4. Interference of SNHG14 ameliorated DA neuron injury induced by rotenone. Primary mesencephalic neurons and MN9D cells were divided into 4 groups: control, Rotenone (Ro, 1μmol/L), Ro+si-control, and Ro+si-SNHG14. (A) The expression of SNHG14 in primary mesencephalic neurons and MN9D cells were measured by qRT-PCR. (B) Cells viability of two kinds of cells were detected by CCK8 assay. (C) Percentage of apoptotic cells in two kinds of cells was quantified using flow cytometry. (D) Relative LDH activity of two kinds of cells. **P<0.01 compared with control; ##P<0.01 compared with Ro+si-control.