Research Paper Volume 11, Issue 21 pp 9264—9279

Dopaminergic neuron injury in Parkinson’s disease is mitigated by interfering lncRNA SNHG14 expression to regulate the miR-133b/ α-synuclein pathway

Figure 5. Direct interactions between SNHG14 and miR-133b in MN9D cells. (A) The potential binding sites between SNHG14 and miR-133b were shown. (B) AGO2-RNA immunoprecipitation (RIP) followed by qRT-PCR was conducted to determine SNHG14 endogenously associated with miR-133b. (C) RNA pull-down assay followed by western blotting determined AGO2 expression pulled down by biotin-labeled SNHG14. The use of biotin-labeled loc285194 was as a positive control for SNHG14. (D) The level of miR-133b in the pull-down complex was detected by qRT-PCR. **P<0.01 compared with IgG; ##P <0.01 compared with loc285194.