Figure 2. Establishment of the TAA-induced cholangiocarcinoma model. (A) Diagram of the DNA insertion site in the loxP alleles of FoxA2. The activation of albumin expression led to the abolishment of FoxA2 expression; (B) Electropherogram of tail DNA genotyping; (C, D) Western blotting and immunohistochemistry were performed to confirm the deletion of FoxA2 expression; (E) Gross observation of liver tissues at the indicated time points (Scale bar: 1 cm). Multiple nodes were detected in FoxA2-/- mice, and the liver / body weight ratio was significantly higher in FoxA2-/- mice; (F) The ALP and ALT levels were higher in FoxA2-/- mice based on the serum biochemistry test. ALP: alkaline phosphatase; ALT: Alanine amino-transferase; ALB: albumin.