Research Paper Volume 11, Issue 21 pp 9280—9294

Loss of FoxA2 accelerates neoplastic changes in the intrahepatic bile duct partly via the MAPK signaling pathway

Figure 3. Loss of FoxA2 promotes the development of intrahepatic bile duct neoplasms and enhances MAPK-related gene expression. (A) Hematoxylin and eosin staining showed remarkable bile duct neoplasm formation in FoxA2-/- mice. Significant liver cirrhosis and proliferation (Ki67 immunohistochemistry) were also observed in the bile duct in FoxA2-/- mice (n=3 each group); (B) Heatmap showing the most differentially expressed genes (DEGs) between FoxA2-/- mice and WT mice. (n = 3 samples per group); (C) KEGG analysis of biological processes. Most of these DEGs were clustered in the “MAPK signaling pathway” category, followed by the “Pathways in cancer”, and “PI13K-AKT signaling pathway”, and so on. The bar indicates the P value; the threshold of P = 0.015 is shown; (D) Volcano plot of P values as a function of the weighted fold change for mRNAs; 805 genes were upregulated genes and 154 were down regulated in FoxA2-/- mice compared with WT mice.; (E) the activation of MAPK-signaling-related genes expression in FoxA2-/- mice.