Figure 5. ROCK2 was a target of miR-682. (A) Thirty-seven common candidates for the targeted mRNAs of miR-682 were found in the Targetscan, miRDB, and microT-CDS database using Venn diagram analysis. VennPlex version 188.8.131.52 software was used for Venn diagram analysis. Proportional circular sets will be drawn if three data sets are uploaded. (B) The binding sites between miR-682 and ROCK2 3’UTR. Luciferase reporter assay was conducted to show the regulation of miR-682 in ROCK2. **P<0.01 vs ROCK2 WT+miRNA. (C) T cells were collected from the spleen in allograft group, allograft+imDEC group and allograft+mDEC group to detect the correlation of miR-682 and ROCK2 mRNA.