Research Paper Volume 11, Issue 20 pp 8911—8924

Immature dendritic cells derived exosomes promotes immune tolerance by regulating T cell differentiation in renal transplantation

Figure 7. imDEC regulated acute rejection after kidney transplantation through miR-682. 10 μg control-sponge-imDEC, miR-682-sponge-imDEC or miR-682 mimic was injected into the allograft model mice via the tail vein 24 hours before and after transplantation (ten mice in each group). Allograft mice were used as NC (n=10). (A) The survival rate in control-sponge-imDEC group, miR-682-sponge-imDEC group, miR-682 mimic group and NC group. (B) H&E staining showed that the remission effect of miR-682-sponge-imDEC on renal inflammation, indicating that imDEC could regulate acute rejection after kidney transplantation through miR-682. (C) Serum IL-2, IL-17 and IFN-γ levels were detected in control-sponge-imDEC group, miR-682-sponge-imDEC group, miR-682 mimic group and NC group.**P<0.01 vs NC; ##P<0.01 vs control-sponge-imDEC. (DG) The proteins of T cells were extracted from the spleen and allograft kidney tissues, and the ROCK2, IL-17 and Foxp3 expressions were detected in control-sponge-imDEC group, miR-682-sponge-imDEC group, miR-682 mimic group and NC group.