Figure 1. Zinc prevented testicular ischemia-reperfusion (I/R) injury in rats. (A) H&E staining of testicular tissues in Control, I/R injury, and I/R injury treated with Zinc rats (×400). Among them, ① Control group (n=8), ② I/R group (n=8), ③ Low-dose Zinc+I/R group (n=8), ④ High-dose Zinc group+I/R (n=8). (B) Mean seminiferous tubular diameter (MSTD) in each group. (C) TUNEL assay of testicular tissues in each group. Apoptotic cells exhibit a brown nuclear stain under microscope (×400). (D) TUNEL positive cells per 103 germ cells and Apoptotic index of testes in each group. Data are shown as mean ± SD. *significant difference vs. Control group (P< 0.05); #significant difference vs. I/R group (P< 0.05).