Research Paper Volume 11, Issue 21 pp 9295—9309

Zinc-induced protective effect for testicular ischemia-reperfusion injury by promoting antioxidation via microRNA-101-3p/Nrf2 pathway

Figure 5. Zinc up-regulated miR-101-3p via Nrf2/HO-1 signaling pathways in TM3 Hypoxia/Reoxygenation (H/R) model. (A) Cell viability of TM3 in Control, H/R injury, and H/R injury treated with Zinc groups. (B) MiRNA-101-3p expression level in H/R with or without Zinc pretreatment groups. (C) The intracellular ROS and Nrf2 levels in H/R with or without Zinc pretreatment groups. (D) Protein levels of Nrf2, NF-κB, HO-1, NQO-1 and GST of TM3 in different groups. Histone H3 and β-actin were used as a protein control to normalize volume of protein expression. (E) The construction diagram of the target genes (Nrf2) double-luciferase reporter genes. (F) The relative luciferase expression with Nrf2 3′-UTR after co-transfection with miR-101-3p mimics or NC mimics in TM3. Data are expressed as mean±SD. *significant difference vs. Control group (P< 0.05); #significant difference vs. H/R group (P< 0.05); **p < 0.01 compared with NC group; ns indicated no significance.