Figure 10. The zebrafish model confirms the inhibitory effect of miR-20b. (A) Observation of retinal tissue sections at 96hpf. We found that in the miRNA-20b mimic group, there were fewer cells in the photoreceptor layer than in the control and wild groups, and the arrangement of cells was disordered. PRL: Photoreceptor layer, ONL: Outer nuclear layer, INL: Inner nuclear layer, GCL: Ganglion cell layer. (B, C) Immunofluorescence detected decreased FGF2 and GRB2 brightness compared to controls. Relative fluorescence values were analyzed using Imagej software (*P<0.05).