Research Paper Volume 11, Issue 20 pp 8969—8981

Expression and significance of miR - 20b in retinal photoreceptor cells exposed to PCB1254

Figure 2. PCB1254 inhibits the expression of related genes. (AC) Total RNA was isolated from 661W cells exposed to different concentrations of PCBs for 24 hours. Photoreceptor cell-specific gene (SWS1, CRX, Rho) expression levels were measured by real-time RT-PCR. The results showed that: As PCB concentration increased, RNA Expression levels gradually decreased (statistically significant in the 1 mg/L group). (D) After 24 hours of exposure, total RNA was isolated from the cells and real-time RT-PCR results showed that: expression of miR-20b was increased with increasing concentrations (0.5mg/L group, 1mg/L group was statistically significant), (EF) Confirmation of miR-20b overexpression and silencing.