Research Paper Volume 11, Issue 20 pp 8969—8981

Expression and significance of miR - 20b in retinal photoreceptor cells exposed to PCB1254

Figure 4. The regulatory relationship between FGF2, GRB2 and miR-20b. (AB) Diagram of 3’UTR sequences of FGF2 and GRB2 targeted by hsa-miR-20b. (CD) HEK293T cells grown to 90% confluency were co-transfected with psiCHECK-2-FGF2 or psiCHECK-2-GRB2 reporter, and the indicated concentration of miR-20b mimics or mimics NC; 24 hours after transfection, luciferase activity was detected using Dual-Luciferase Reporter Assay System according to the manufacturer’s instruction.