Figure 9. Effect of mir-20b on zebrafish embryos. (A) Injection of 4uM dre-miRNA-20b mimic and NC simulation into zebrafish embryos, embryo morphology at different time points. (B) Total RNA isolated from 96h post-injection embryos was used to determine FGF2 and GRB2 levels by real-time RT-PCR. The results showed that RNA expression was reduced compared to the control group.