Figure 6. Baicalein suppresses continuously OVA challenge induced collagen deposition. (A) Lung tissue sections were stained with Sirius Red to assess collagen deposition. Images are shown at 200× magnification with a scale bar representing 100 μm. (B) A bar graph showing quantified collagen deposition areas (%) detected by Sirius Red staining. (C–D) Expression levels of collagen I (Col I) and MMP9 in the lung tissues of mice in each group were determined by RT-qPCR. Lung tissues from each group were extracted for western blotting to analyze collagen I and MMP-9 expression, with β-actin used as a loading control. Proteins from three mouse lung tissues were pooled together, n = 6 in one group (F–G). The results are presented as the mean ± SEM; ##P < 0.01 compared with the control group; *P < 0.05, **P < 0.01 compared with the OVA/Vehicle group.