Research Paper Volume 11, Issue 21 pp 9310—9327

Baicalein attenuates OVA-induced allergic airway inflammation through the inhibition of the NF-κB signaling pathway

Figure 7. Baicalein inhibits OVA-induced NF-κB pathway activation. (A) The protein levels of P-IκBα and IκBα in the lung tissues of mice in each group were examined by western blot analysis with β-actin used as an internal control. (BC) A bar graph shows the quantification of P-IκBα, IκBα and β-actin by densitometry. (DF) Cytosolic (upper blot) and nuclear (lower blot) p65 levels were determined by western blot. β-actin and Lamin B were used as loading controls. Proteins from three mouse lung tissues were pooled together. n = 6 in one group. (G) Immunofluorescence staining for p65 (red, Cy3) in lung tissues of mice at 100× magnification. Nuclei were stained with DAPI (blue). (H) Relative nuclear immunostaining intensity of p65 was quantified. The results are presented as the mean ± SEM. n = 6 mice per group; ##P < 0.01 compared with the control group; *P < 0.05, **P < 0.01 compared with the OVA/Vehicle group.