Research Paper Volume 11, Issue 21 pp 9310—9327

Baicalein attenuates OVA-induced allergic airway inflammation through the inhibition of the NF-κB signaling pathway

Figure 8. iNOS expression and activity are suppressed upon Baicalein treatment. (A) The expression of iNOS in the total proteins of lung tissues from mice in each group was detected by western blot analysis, and β-actin was used as an internal control. Proteins from three mouse lung tissues were pooled together. n = 6 in one group. (B) A bar graph shows the quantification of iNOS and β-actin by densitometry. Total NO synthase (TNOS, C) and inducible NO synthase (iNOS, D) activities in mouse serum were measured using an NOS Assay Kit (Error bars represent the mean ± SEM; ##P < 0.01 compared with the control group; **P < 0.01 compared with the OVA/Vehicle group).