Figure 1. LPS promotes BV-2 cell death by downregulating Sirt3. (A) BV-2 cell viability was measured after exposure to different doses of LPS. (B) Sirt3 transcript levels were measured using qPCR after exposure to different concentrations of LPS. (C) An LDH release assay was used to evaluate cell death in response to LPS treatment. Sirt3 adenovirus was transfected into BV-2 cells to overexpress Sirt3. (D) Cell apoptosis was determined by analyzing the activity of caspase-3 using ELISA in BV-2 cells overexpressing Sirt3. (E, F) TUNEL staining was used to measure the cell death after exposure to LPS in BV-2 cells overexpressing Sirt3. *P<0.05 vs. control group; #P<0.05 vs. LPS+adenovirus-control group. N=3 independent experiments.