Figure 2. Sirt3 overexpression attenuates LPS-mediated mitochondrial damage in BV-2 cells. (A, B) Mitochondrial membrane potential was measured by analyzing red-to-green fluorescence intensity ratios for the JC-1 probe. (C, E) An ELISA assay was used to evaluate alterations in the mitochondrial respiratory complex of BV-2 cells after exposure to LPS stress. (F, G) Mitochondrial state-3 and state-4 respiration were measured by ELISA. BV-2 cells were treated with LPS and/or transfected with Sirt3 adenovirus. *P<0.05 vs. control group; #P<0.05 vs. LPS+adenovirus-control group. N=3 independent experiments.