Figure 4. Fructus Ligustri Lucidi (FLL) decreases Nox4 expression and modulates the impaired gut microbiota in aging mice. (A) The representative western blot image and their analysis showed tibias Nox4 expression. (B) Shannon and Simpson diversity indices. (C) Chao 1 and ACE diversity indices. (D) Relative abundances of bacterial operational taxonomy units (OTUs) at the phylum level. LEfSe cladograms (from kingdom to genus) (E) and Linear discriminant analysis (LDA)-derived histogram of differentially abundant taxa (F). Relative abundances of major bacterial OTUs in class (G), order (H), family (I), and genus (J) level. Data are presented as mean ± SD. # compared with the normal group. * compared with the aging model group. p < 0.05 was considered statistical difference, n=5.