Figure 6. Fructus Ligustri Lucidi (FLL) increases tibial and femoral Sirt6 expression and regulates serum levels of TNF-α and IL-2 in aging mice. Immunohistochemical staining and western blot showed Sirt6 expression (original magnification, ×200, ×400) in the femoral metaphysis (A–B) and femoral heads (C–D), and in the tibias (E) of different groups of mice. Serum levels of IL-2 (F) and TNF-α (G). Data are presented as mean ± SD. # compared with the normal group. * compared with the aging model group. p < 0.05 was considered statistical difference, n=8.