Research Paper Volume 11, Issue 21 pp 9348—9368

Fructus Ligustri Lucidi preserves bone quality through the regulation of gut microbiota diversity, oxidative stress, TMAO and Sirt6 levels in aging mice

Figure 6. Fructus Ligustri Lucidi (FLL) increases tibial and femoral Sirt6 expression and regulates serum levels of TNF-α and IL-2 in aging mice. Immunohistochemical staining and western blot showed Sirt6 expression (original magnification, ×200, ×400) in the femoral metaphysis (AB) and femoral heads (CD), and in the tibias (E) of different groups of mice. Serum levels of IL-2 (F) and TNF-α (G). Data are presented as mean ± SD. # compared with the normal group. * compared with the aging model group. p < 0.05 was considered statistical difference, n=8.