Figure 2. Overexpression of ARHGAP27P1 inhibited proliferation, cell cycle progression, invasion and migration, while induced apoptosis of GC cells. (A) RT-qPCR analysis of ARHGAP27P1 expression in GES-1 cells and different GC cells. (B) Relative ARHGAP27P1 levels in SGC-7901 and AGS cells transfected with pcDNA or pcDNA-ARHGAP27P1. (C) Cell proliferation in SGC-7901 and AGS cells transfected with pcDNA or pcDNA-ARHGAP27P1 was determined by cell counting assays. (D) Colony formation assays. (E) Cell apoptosis assays. (F) Cell cycle assays. (G) Wound-healing assays. (H) Transwell migration assays. (I) Transwell invasion assays. *P < 0.05, **P < 0.01, ***P < 0.001 versus GES-1 or vector.