Research Paper Volume 11, Issue 20 pp 9090—9110

Long noncoding RNA ARHGAP27P1 inhibits gastric cancer cell proliferation and cell cycle progression through epigenetically regulating p15 and p16

Figure 2. Overexpression of ARHGAP27P1 inhibited proliferation, cell cycle progression, invasion and migration, while induced apoptosis of GC cells. (A) RT-qPCR analysis of ARHGAP27P1 expression in GES-1 cells and different GC cells. (B) Relative ARHGAP27P1 levels in SGC-7901 and AGS cells transfected with pcDNA or pcDNA-ARHGAP27P1. (C) Cell proliferation in SGC-7901 and AGS cells transfected with pcDNA or pcDNA-ARHGAP27P1 was determined by cell counting assays. (D) Colony formation assays. (E) Cell apoptosis assays. (F) Cell cycle assays. (G) Wound-healing assays. (H) Transwell migration assays. (I) Transwell invasion assays. *P < 0.05, **P < 0.01, ***P < 0.001 versus GES-1 or vector.