Research Paper Volume 11, Issue 20 pp 9090—9110

Long noncoding RNA ARHGAP27P1 inhibits gastric cancer cell proliferation and cell cycle progression through epigenetically regulating p15 and p16

Figure 3. Silencing of ARHGAP27P1 promoted proliferation, cell cycle progression, invasion and migration, and inhibited apoptosis of HGC-27 cells. Scramble siRNA (si-NC) or ARHGAP27P1 siRNA (si-ARHGAP27P1) was transfected into HGC-27 cells. (A) Knockdown efficiency of si-ARHGAP27P1 was determined by RT-qPCR. (B) Cell proliferation was determined by cell counting assays in ARHGAP27P1-silencing HGC-27 cells. (C) Colony formation assays. (D) Cell apoptosis assays. (E) Cell cycle assays. (F) Wound healing assays. (G) Transwell migration and invasion assays. *P < 0.05, **P < 0.01 versus si-NC.