Figure 4. ARHGAP27P1 affected the expression of p15, p16 and p57. (A) RT-qPCR analysis of the main CKIs including p15, p16, p21, p27 and p57 in HGC-27 cells transfected with si-ARHGAP27P1 or si-NC. (B and C) RT-qPCR analysis of the main CKIs in SGC-7901 or AGS transfected with pcDNA-ARHGAP27P1 or pcDNA. (D–F) Spearman’s correlation analysis of the relationship between the expression of ARHGAP27P1 and that of p15, p16 or JMJD3 in 24 GC tissues. (G) Western blot analysis of JMJD3, H3K27me3, p15, p16 and p57 in 5 cases of cancerous tissues with high ARHGAP27P1 expression and 5 cases with low ARHGAP27P1 expression. *P < 0.05 versus si-NC, vector or low ARHGAP27P1 expression.