Figure 2. Senescent NPCs’ ECM degradation and cell cycle suppression been alleviated in BMSCs coculture model. (A) Immunofluorescence staining of MMP9 combined with DAPI staining for nuclei. n=5, Scale bar, 50um. (B) MMP9 fluorescence intensities were determined by using Image J software. (C–D) Flow cytometry results between sene +BMSCs and sene +NPCs. n=3 (E) The mRNA expressions of p16Ink4a, p21 and p53 were measured by real time PCR. n=3. (F–G) The protein expressions of P16, P21 were visualized by western blot and quantified by Image J. Values represent means±S.D. Significant differences between different groups are indicated as *P < 0.05, **P < 0.01.