Research Paper Volume 11, Issue 20 pp 9167—9187

Bone marrow-derived mesenchymal stem cells in three-dimensional co-culture attenuate degeneration of nucleus pulposus cells

Figure 2. Senescent NPCs’ ECM degradation and cell cycle suppression been alleviated in BMSCs coculture model. (A) Immunofluorescence staining of MMP9 combined with DAPI staining for nuclei. n=5, Scale bar, 50um. (B) MMP9 fluorescence intensities were determined by using Image J software. (CD) Flow cytometry results between sene +BMSCs and sene +NPCs. n=3 (E) The mRNA expressions of p16Ink4a, p21 and p53 were measured by real time PCR. n=3. (FG) The protein expressions of P16, P21 were visualized by western blot and quantified by Image J. Values represent means±S.D. Significant differences between different groups are indicated as *P < 0.05, **P < 0.01.