Figure 4. Correlation between ZMPSTE24 and TNF-α induced RelA activation. (A, C, D) Protein expression of RelA with stable empty vector (SC), ZMPSTE24 overexpression or knockout of ZMPSTE24 were visualized by western blot and quantified by Image J. n=3. (B) The nuclear translocation of RelA was detected by immunofluorescence combined with DAPI staining for nuclei. n=5. Scale bar: 20 μm. (E–F) Immunofluorescence staining of MMP9 combined with DAPI staining for nuclei. n=5, Scale bar, 50um. Values represent means±S.D. Significant differences between different groups are indicated as *P < 0.05, **P < 0.01, ***P < 0.001.