Research Paper Volume 11, Issue 20 pp 9209—9219

Overexpression of steroid sulfotransferase genes is associated with worsened prognosis and with immune exclusion in clear cell-renal cell carcinoma

Figure 4. In vitro assays showing functional analysis of SULT genes in ccRCC. (A) shown are efficacies of shRNAs targeting SULT genes in 786-O cells; (B) Proliferation at 72 hours of culture detected by MTT assay in 786-O cells; (C) shown was efficacy of lentiviral delivery of SULT1B1 overexpression; Shown were overexpression and silencing of SULT2B1 impacting on (D) proliferation by MTT, (E) cell cycle population and (F) apoptosis by flow cytometry, (G) migration and invasion by Transwell assay, and (H) colony formation assay (n = 4, *P < 0.05; **P < 0.01).