Research Paper Volume 11, Issue 21 pp 9442—9460

Long noncoding RNA DNM3OS promotes prostate stromal cells transformation via the miR-29a/29b/COL3A1 and miR-361/TGFβ1 axes

Figure 3. DNM3OS silencing decreases the protein level of COL3A1 (A) DNM3OS silencing conducted in PrSCs by transfection of si-DNM3OS#1 or si-DNM3OS#2 and confirmed by real-time PCR. PrSCs were transfected with si-DNM3OS in the presence or absence of TGFβ1 and examined for (B) the protein level of COL3A1 by Immunoblotting and (C) the protein content and distribution of COL3A1 by immunofluorescence (IF) staining (scale bar: 20 μM). *P<0.05, **P<0.01 compared to the si-NC group, ## P<0.01 compared to si-NC+TGFβ1 group.