Research Paper Volume 11, Issue 21 pp 9442—9460

Long noncoding RNA DNM3OS promotes prostate stromal cells transformation via the miR-29a/29b/COL3A1 and miR-361/TGFβ1 axes

Figure 7. The dynamic effects of DNM3OS and miR-361 on TGFβ1 and downstream signaling PrSCs were cotransfected with si-DNM3OS and miR-361 inhibitor and examined for (A) the protein content and distribution of α-SMA by IF staining (scale bar: 50 μM); (B) the protein levels of α-SMA and ACTG2 by immunoblotting and (C) the protein levels of TGFβ1, p-Smad2, and Smad2 by immunoblotting.