Figure 5. CAT ameliorated liver steatosis by inducing autophagy via AMPK. Cells were treated with 0.3 mM PA and 10 μg/mL CAT for 24 h in the presence or absence of 50 mM chloroquine (CQ) for 2 h. HepG2 cells were stained with Oil Red O (A), and intracellular TG and TC were quantitatively analyzed (B). Scale bars: 25 μm. HepG2 cells treated with 0.3 mM PA, 10 μg/mL CAT for 24 h, and 10 μM compound C (CC) for 24 h. Cells were stained with Oil Red O (C), and intracellular TG and TC were quantitatively analyzed (D). Scale bars: 25 μm. (E) Expression of hepatic lipogenic genes ACC1α and FAS and fatty acid oxidation genes PPARα and CPT1 was quantitatively analyzed (n=3). (F) A schematic illustration demonstrating the role of CAT in NAFLD and the effect of CAT on autophagy. *P < 0.05, **P < 0.01, ***P < 0.001.