Research Paper Volume 11, Issue 21 pp 9530—9543

Proteome and phosphoproteome reveal mechanisms of action of atorvastatin against esophageal squamous cell carcinoma

Figure 5. Atorvastatin inhibits phosphorylation of ERKT187/Y187, CDK1T14, BRCA1S1189and induces G0/G1 arrest in ESCC cells. (A) shows CDK1T14, ERKT185/Y187, and BRCA1S1189 are down-regulated in the phosphorylation profile. (B) Western blotting of atorvastatin-treated KYSE150 cells verifies the results above. (C) With increasing concentration of atorvastatin (0, 1, 2.5, 5, 10 and 20 μM), the levels of ERKT185/Y187, CDK1T14, and BRCA1S1189 are decreased. (D) The effects of atorvastatin on cell cycle phase were assessed in KYSE150 cells. Cells were treated with 0, 1, 2.5, and 5 μM atorvastatin and then incubated 48 h. The asterisks (* P < 0.05, ** P < 0.01, *** P < 0.001) indicate a significant difference between untreated control and atorvastatin-treated cells.