Figure 3. Sig-1R and GRP78 protein expression on the ER after CIRI. (A) The protein levels of Sig-1R and GRP78 were determined by Western blotting. (B) Sig-1R protein levels with increasing reperfusion times. (C) GRP78 protein levels with increasing reperfusion times. The results were normalized to the percentage of Calnexin expression. Data are shown as the mean ± SEM, n = 6 per group. #P < 0.05, ##P < 0.01 compared with the MCAO 0-hour group.