Research Paper Volume 11, Issue 21 pp 9556—9568

Dexmedetomidine inhibits neuronal apoptosis by inducing Sigma-1 receptor signaling in cerebral ischemia-reperfusion injury

Figure 4. The effects of dexmedetomidine on the expression of Sig-1R and ERS-induced apoptotic proteins after CIRI. (A) The protein levels of Sig-1R in the cerebral ischemic penumbra were determined by Western blotting. (B) The levels of ERS-induced apoptotic proteins (CHOP, p-JNK/JNK, Caspase-3) in the cerebral ischemic penumbra were determined by Western blotting. (C) Sig-1R protein expression. (D) CHOP protein expression. (E) p-JNK/JNK protein expression. (F) Caspase-3 protein expression. The results were normalized to the percentage of Calnexin or β-actin expression. Data are shown as the mean ± SEM, n = 6 per group. *P < 0.05, **P < 0.01 compared with the sham group; #P < 0.05, ##P < 0.01 compared with the MCAO group. Sham operation group, Sham group; dexmedetomidine administration group, Dex group; MCAO model control group, MCAO group; MCAO + dexmedetomidine administration group, MCAO + Dex group.