Research Paper Volume 11, Issue 21 pp 9556—9568

Dexmedetomidine inhibits neuronal apoptosis by inducing Sigma-1 receptor signaling in cerebral ischemia-reperfusion injury

Figure 5. The effects of dexmedetomidine on the ERS signaling pathway involving Sig-1R after CIRI. (A) GRP78 protein levels on the ER were determined by Western blotting. (B) The ERS-induced apoptotic proteins (CHOP, Caspase-3) from the cerebral ischemic penumbra were determined by Western blotting. (C) GRP78 protein levels. (D) CHOP protein levels. (E) Caspase-3 protein levels. The results were normalized to the percentage of Calnexin or β-actin expression. Data are shown as the mean ± SEM, n = 6 per group. #P < 0.05, ##P < 0.01 compared with the MCAO group; P < 0.05, ☆☆P < 0.01 compared with the MCAO + dexmedetomidine group. MCAO + BD1063 group, MCAO + BD group; MCAO + dexmedetomidine group, MCAO + Dex group; MCAO + BD1063 + dexmedetomidine group, MCAO + BD + Dex group.