Research Paper Volume 11, Issue 21 pp 9569—9580

TGF-β- and lipopolysaccharide-induced upregulation of circular RNA PWWP2A promotes hepatic fibrosis via sponging miR-203 and miR-223

Figure 1. Screening of circRNAs. (A) Human HSC cell line LX-2 was activated by TGF-β (5 ng/ml) or LPS (5 μg/ml). After 24 hours, the expressions of several circRNAs, including hsa_circ_0072765, hsa_circ_0071410, hsa_circ_0054345, hsa_circ_0070963, hsa_circ_0061893, hsa_circ_0013255, hsa_circ_0074837 (the circ-PWWP2A in human genome), and hsa_circ_0072758, were detected in activated HSCs using qRT-PCR. (B) The fibrosis mouse model was established by i.p. injection of olive oil-dissolved CCl4 (0.3 ml/kg) thrice a week for 6 weeks. Liver tissues from the control group (n=7) and the CCl4 group (n=7) were collected for HE staining and the expression of mmu_circ_0000254 (the circ-PWWP2A in mouse genome) was detected using qRT-PCR. Magnification is 400X. Scale bar represents 200 μM. **p<0.01 vs control.