Figure 3. PVT1 promotes ERG expression via sponging miR-183-5p. (A) The online database predicted the potential binding between PVT1 and miR-183-5p. (B) The luciferase activity after the co-transfection of miR-183-5p mimic and pGL3-PVT1-wt or miR-183-5p mimic and pGL3-PVT1-mut. (C) The expression of miR-183-5p in PVT1-interfering osteosarcoma cells. (D) The online database predicted the interaction between miR-183-5p and ERG 3’-UTR. The luciferase activity of ERG 3’-UTR and the protein level of ERG were detected after overexpressing miR-183-5p. (E) The ERG protein expression after the transfection of si-PVT1 or the co-transfection with miR-183-5p inhibitor for 48 h in three osteosarcoma cell lines. Three independent experiments. **p<0.01 vs negative control (pre-NC) or si-control. wt, wild type. mut, mutant. si-PVT1, the siRNA of PVT1. Pre-NC, the control of miR-183-5p mimic. NC, the control of imR-183-5p inhibitor.