Research Paper Volume 11, Issue 21 pp 9626—9642

Cellular senescence induced by S100A9 in mesenchymal stromal cells through NLRP3 inflammasome activation

Figure 5. NLRP3 overexpression induces IL-1β secretion and cellular senescence. (A) The efficiency of NLRP3 overexpression was assayed with qPCR and western blot. (B) Senescent cells were counted and visualized in graphs (40 × magnification). The levels of p53, p21, p16, and IL-1β were measured by qPCR and western blot. Data are expressed as mean ± SD from three experiments. *P<0.05, **P<0.01, and ***P<0.001.