Research Paper Volume 11, Issue 21 pp 9643—9660

Oncogenic USP22 supports gastric cancer growth and metastasis by activating c-Myc/NAMPT/SIRT1-dependent FOXO1 and YAP signaling

Figure 3. USP22 knockdown induces in vitro GC cell apoptosis. The results of (A) Annexin V/PI staining, (B) TUNEL, and (C) caspase-3 activity assays in BGC-823 and HGC-27 cells that are untransfected (Mock) or transfected with 100 nM siNC or siUSP22-1are shown. Note: Data are expressed as mean ± SD of three replicates; *p < 0.05 compared with the mock or siNC group.