Research Paper Volume 11, Issue 21 pp 9672—9688

Glycogen synthase kinase-3β suppresses the expression of protein phosphatase methylesterase-1 through β-catenin

Figure 4. β-catenin enhances PME-1 expression. (A) β-Catenin or siβ-catenin was transfected together with pGL4/PME-1-350 and pRL-TK into HEK-293T cells, and then the PME-1 expression was determined through luciferase activity. (B) HEK-293T cells were co-transfected with pGL4/PME-1s, β-catenin and pRL-TK. The luciferase activity was measured. (CE) HEK-293T cells were transfected with β-catenin or siβ-catenin and analyzed for PME-1 and β-catenin by Western blots (C, D) or qPCR (E). Quantification (E) of the Western blots are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.