Research Paper Volume 11, Issue 21 pp 9689—9708

Circular RNA circGRAMD1B inhibits gastric cancer progression by sponging miR-130a-3p and regulating PTEN and p21 expression

Figure 8. circGRAMD1B inhibits GC growth and metastasis in vivo. (A) Representative images of xenograft tumors induced by circGRAMD1B overexpressing vector and control vector-transfected MGC803 cells (five mice per group). (B) A growth curve of the tumor growth in circGRAMD1B overexpressing vector and control vector-transfected MGC803 cells. (C) Tumor weights of the xenograft tumors in circGRAMD1B overexpressing vector and control vector-transfected MGC803 cells. (D) Expressions levels of circGRAMD1B, PTEN, p21, Ki-67, PCNA, E-cadherin, and Vimentin in xenograft tumors were determined by qRT-PCR assays. (E) Expression levels of PTEN, p21, Ki-67, PCNA, E-cadherin, and Vimentin were shown by IHC staining in representative xenograft tumors. (F) Representative images of lung metastatic nodules in the circGRAMD1B overexpression group and control group are indicated by white arrows. (G) The number of tumor nodules on lung surfaces from the circGRAMD1B overexpression group and control group. (H) qRT-PCR was used to detect the expression levels of circGRAMD1B, Ki-67, PCNA, E-cadherin, and Vimentin in lung metastasis from nude mice in the circGRAMD1B overexpressing group and control group. (Values are shown as the mean ± standard error of the mean based on three independent experiments. *P < 0.05, **P < 0.01).