Research Paper Volume 11, Issue 21 pp 9767—9777

Resistin enhances angiogenesis in osteosarcoma via the MAPK signaling pathway

Figure 5. Effects of resistin on VEGF-A-induced angiogenesis in the CAM model. (A) PBS, VEGF-A (50 ng/ml), control osteosarcoma 143B cell CM and resistin-treated osteosarcoma 143B CM were mixed in Matrigel and subjected to the CAM assay, then photographed with a stereomicroscope on developmental day 12. (B) Angiogenesis was quantified by counting the number of blood vessel branches. * p < 0.05; ** p < 0.01; *** p < 0.001 compared with controls.