Research Paper Volume 11, Issue 21 pp 9794—9810

LHX2 promotes malignancy and inhibits autophagy via mTOR in osteosarcoma and is negatively regulated by miR-129-5p

Figure 5. LHX2 is directly targeted by miR-129-5p in OS cells. (A) miR-129-5p expression in OS cell lines determined by qPCR. *P <0.05. (B) miR-129-5p binding was predicted in the 3’UTR of LHX2 using the informatic tool Targetscan. (C) Dual-luciferase reporter assays were performed in 143B and MG63 cells cotransfected with putative or mutant LHX2 3’UTR-luciferase reporters and lv-miR-129-5p. *P <0.05, **P<0.01. (D) Corresponding expression of miR-129-5p for 143B and MG63 cells infected with lv-miR-129-5p or lv-Anti-miR-129-5p. *P <0.05, **P<0.01. (E) LHX2 expression in OS cells infected with lv-miR-129-5p or lv-Anti-miR-129-5p.