Research Paper Volume 11, Issue 21 pp 9794—9810

LHX2 promotes malignancy and inhibits autophagy via mTOR in osteosarcoma and is negatively regulated by miR-129-5p

Figure 6. LHX2 restoration partially reverses miR-129-5p overexpression-mediated malignant phenotypes in vitro. (AB) CCK8 and colony formation assays indicated that the inhibitory effects of miR-129-5p can be partially reversed by LHX2 overexpression. **P<0.01. © Ki67 expression in shLHX2 and control groups were determined by western blot. (D) Represent imaging of wound healing assays and cell migration rates were measured after 24 h. Scale bar: 100 μm. (E) Represent images of transmembrane cells in shLHX2 and control groups. *P <0.05, **P<0.01. Scale bars: 200 μm. (F) Cell migration rates and the number of transmembrane cells.