Research Paper Volume 11, Issue 21 pp 9811—9828

The TORC1 inhibitor Nprl2 protects age-related digestive function in Drosophila

Figure 4. Mutant one copy of Tor rescued the defects in digestive function in nprl2 mutants. Fifteen-day-old yw, nprl21, and nprl21; TorA594V/+ males were used for the following analyses. (A and B) The ratios of crop size and crops with food content were quantified as described in Figure 2. Error bars represent the SD from three independent experiments. (C and D) Flies were cultured in dyed food for 3 days and the dyed food concentration in the crop and gut was determined using a spectrophotometer. Error bars represent the SD of the indicated number of data points. (E) Flies were cultured on blue dyed food for 3 days and then transferred to standard food. The ratio of flies with blue dye in the digestive tube was calculated. Error bars represent the SD from four independent experiments. (F) Contractions of the crop were measured in dissected flies. Error bars represent the SD of the indicated number of data points. NS, not significant. ***P < 0.001. N is the total number of flies used.