Figure 6. nprl2 mutation affected ISCs and EE cells in the midgut. (A–C) Newly hatched (A) yw; esg-Gal4, UAS-GFP, tub-Gal80ts, (B) nprl21; esg-Gal4, UAS-GFP, tub-Gal80ts and (C) nprl21; esg-Gal4, UAS-GFP, tub-Gal80ts / TorA594V flies were cultured on standard food for 15 days at 18°C, then transferred to 29°C and cultured for a further 3 days. Flies were dissected and stained with anti-Delta antibody. (D–F) Fifteen-day-old (D) yw, (E) nprl21 and (F) nprl21; TorA594V/+ flies were dissected and stained with an anti-Prospero antibody. (G) The number of Esg+ cells in the posterior midgut (PMG) was counted. (H) The size of Esg+ cells in the PMG was measured. (I) The number of Prospero+ cells in the PMG was counted. Error bars represent the SD of the indicated number of data points. *P < 0.05, **P < 0.01, ***P < 0.001.