Research Paper Volume 11, Issue 21 pp 9829—9845

Slower rates of accumulation of DNA damage in leukocytes correlate with longer lifespans across several species of birds and mammals

Figure 1. DNA damage γH2AX measurements for various species. The level of γH2AX was measured by immunofluorescence in leukocytes in a high-throughput manner in individuals of different ages for (A) bottlenose dolphins (Tursiops truncatus), (B) goats (Capra hircus), (C) reindeer (Rangifer tarandus), (D) American flamingos (Phoenicopterus ruber), (E) griffon vultures (Gyps fulvus). Each point represents the values for a different individual. The correlation coefficient (R2), slope (rate of γH2AX increase in % positive cells per year), and y-intercept are presented on the graphs. Representative images show cell nuclei stained with DAPI in blue and yH2AX stain in green for a young individual and an older individual for each species. White arrows indicate γH2AX positive cells.