Figure 3. Insulin, IGF-1 and IGFBPs circulation levels. The insulin levels in male (A) and female (B) Dom and Sub mice at the age of 12 weeks (Dom males n=5, females n=5; Sub males n=5, females n=6) and 26 weeks (Dom males n=5, females n=4; Sub males n=5, females n=5). In 26-week-old Sub males the fasting insulin level was significantly lower than in Dom (t=3.803, p<0.01). (C) IGF-1, IGFBP-2, IGFBP-3 and IGFBP-6 serum levels in Dom and Sub male mice. In the serum of Sub mice a significant elevation of IGF-1 (t=20.11, p<0.01) and a significant reduction of its binding proteins IGFBP-2 (t=17.21, p<0.01), IGFBP-3 (t=6.474, p<0.05) and IGFBP-6 (t=4.569, p<0.05) was detected (n=5 per group). * - p<0.05, ** - p<0.01. Student unpaired two-tailed t-test was used. Error bars indicate SEM.